Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Targeting the IL-15 Receptor with an Antagonist IL-15 Mutant/Fc?2a Protein Blocks Delayed-Type Hypersensitivity 1

doi:

Figure Lengend Snippet: IL-15 mutant/Fcγ2a protein has prolonged circulating t1/2 (6 h). The time-related serum concentration was determined following a single bolus i.v. dose (6 µg) of the fusion protein. Blood samples were obtained by retroorbital bleeding at the indicated intervals. The levels of mutant fusion protein were detected by ELISA with rat anti-human IL-15 Ab as capture Ab and horseradish peroxidase-conjugated rat anti-mouse IgG2a Ab as the detection Ab. Four mice were used for determining the t1/2. Data = mean ± SD.

Article Snippet: The secondary Ab used for all immunostaining was rabbit anti-rat IgG conjugated with biotin (Vector Laboratories).

Techniques: Mutagenesis, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Targeting the IL-15 Receptor with an Antagonist IL-15 Mutant/Fc?2a Protein Blocks Delayed-Type Hypersensitivity 1

doi:

Figure Lengend Snippet: Immunohistochemical staining for the presence of macrophages/monocytes (12 h after Ag rechallenge) with anti-mouse F4/80 IgG2b. The arrows denote for F4/80+ cells in specimens obtained from mice undergoing: mouse IgG treatment (a), IL-15 mutant/Fcγ2a protein treatment (b), mouse IgG and CsA treatment (c), and IL-15 mutant/Fcγ2a and CsA treatment (d). The decrease in footpad swelling and cell infiltration in IL-15 mutant/Fcγ2a ± CsA-treated groups is parallel.

Article Snippet: The secondary Ab used for all immunostaining was rabbit anti-rat IgG conjugated with biotin (Vector Laboratories).

Techniques: Immunohistochemical staining, Staining, Mutagenesis